Pumpkin is one of the best dietary sources of carotenoid, the pigment responsible for the yellow, orange and red colours in plants. Carotenoids are important antioxidants and those found in pumpkin can be converted to vitamin A, important for growth and development, immune system function and vision. beta-Carotene is the main constituent of pumpkin yellow pigments. The orange colour of pumpkin signals beta carotene. In addition, this plant also contains other pigments, like alpha-carotene, gamma-carotene, zeaxanthin, lycopene and xanthophyll, etc. Pumpkin pigments are extracted based on Cucurbita maschata D by modern biotechnology. The extraction processes of this yellow pigment have been studied. Pumpkin yellow pigment is used as a natural yellow colorant in margarine, ice cream, ice-lolly, fruit juice and biscuits, etc.